Chlorate-resistant, Nitrate-utilizing (crn) Mutants of Fusarium moniliforme (Gibberella fujikuroi)
نویسندگان
چکیده
منابع مشابه
Nitrate reduction mutants of fusarium moniliforme (gibberella fujikuroi).
Twelve strains of Fusarium moniliforme were examined for their ability to sector spontaneously on toxic chlorate medium. All strains sectored frequently; 91% of over 1200 colonies examined formed chlorate-resistant, mutant sectors. Most of these mutants had lesions in the nitrate reduction pathway and were unable to utilize nitrate (nit mutants). nit mutations occurred in seven loci: a structur...
متن کاملMolybdenum cofactor in chlorate-resistant and nitrate reductase-deficient insertion mutants of Escherichia coli.
We examined molybdenum cofactor activity in chlorate-resistant (chl) and nitrate reductase-deficient (nar) insertion mutants and wild-type strains of Escherichia coli K-12. The bacterial molybdenum cofactor was assayed by its ability to restore activity to the cofactor-deficient nitrate reductase found in the nit-1 strain of Neurospora crassa. In the wild-type E. coli strains, molybdenum cofact...
متن کاملNew PCR Assays for the Identification of Fusarium verticillioides, Fusarium subglutinans, and Other Species of the Gibberella fujikuroi Complex
Fusarium verticillioides and Fusarium subglutinans are important fungal pathogens of maize and other cereals worldwide. In this study, we developed PCR-based protocols for the identification of these pathogens targeting the gaoB gene, which codes for galactose oxidase. The designed primers recognized isolates of F. verticillioides and F. subglutinans that were obtained from maize seeds from sev...
متن کاملRegulation of Gibberellin Biosynthesis in Gibberella fujikuroi.
Gibberellin production by Gibberella fujikuroi started only after the nitrogen source was depleted and ceased upon its renewal. Nitrogen repression of gibberellin biosynthesis is not an indirect effect of the growth arrest that follows the depletion of an essential nutrient because gibberellins were not produced upon depletion of phosphate. Mycelia produced gibberellins when suspended in a gluc...
متن کاملIsozyme Variation among Biological Species in the Gibberella fujikuroi Species Complex (Fusarium Section Liseola).
Isozyme phenotypes were determined for 101 strains of Gibberella fujikuroi and 2 strains of Gibberella nygamai that represent seven biological species (mating populations) isolated from a variety of plant hosts in dispersed geographic locations. Fourteen enzymes were resolved in one or more of three buffer systems. Two of the enzymes, arylesterase and acid phosphatase, were polymorphic within t...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1989
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-135-3-721